Research Resources
Reconstitution Guide
A practical reference for reconstituting lyophilised peptides in a laboratory setting. This guide is for research purposes only.
Research use only
Professor Peptide products are strictly for laboratory research. They are not intended for human or veterinary consumption, diagnosis, or treatment. Always follow your institutional safety protocols and local regulations.
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Step-by-step protocol
Bring the vial to room temperature
Allow the lyophilised peptide to sit at room temperature for 10–15 minutes before opening. Cold vials can draw moisture from the air.
Wipe the septum with an alcohol swab
Disinfect the rubber stopper on the peptide vial and the bacteriostatic water vial. Let the alcohol evaporate fully.
Draw the diluent slowly
Use a sterile research syringe to draw the required volume of bacteriostatic water. Choose a volume that gives a convenient working concentration.
Inject down the inside wall
Insert the needle at a shallow angle and dispense the water slowly down the inner glass wall. Do not shoot directly onto the powder to avoid foaming.
Dissolve without shaking
Gently swirl the vial until the powder is fully dissolved. Never shake vigorously. Most peptides dissolve within 30–60 seconds.
Label and store correctly
Mark the vial with the compound, concentration, date and solvent. Store reconstituted peptides at 2–8 °C for short-term use, or aliquot and freeze at -20 °C.
Quick reference
Solvent
Bacteriostatic water (0.9% benzyl alcohol)
Working temp
Room temperature before opening
Refrigerated shelf life
2–4 weeks at 2–8 °C
Frozen shelf life
Months at -20 °C in aliquots
Avoid
Tap water, repeated freeze/thaw, direct spray
Light
Protect from bright light and UV sources
Frequently asked questions
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