Research Guide · Updated June 2026
How to Reconstitute Peptides — UK Research Guide
Reconstitution turns a lyophilised research peptide into a liquid for downstream in-vitro assays. The procedure is simple but sterility and accurate volume measurement matter.
What you need
Lyophilised peptide vial. Bacteriostatic water (BAC water) — available as an ancillary on our store. A sterile syringe with fine-gauge needle. Alcohol swabs. A clean lab bench.
Step-by-step procedure
1. Allow both vials to reach room temperature. 2. Wipe the rubber stoppers of both vials with an alcohol swab. 3. Draw the required volume of BAC water into the syringe (see our dose calculator). 4. Inject the BAC water down the inside wall of the peptide vial — do not squirt directly onto the lyophilised powder. 5. Swirl gently to dissolve; do not shake. 6. Once fully dissolved, store at 2–8 °C and use within the stability window for the compound class.
Common mistakes
Shaking instead of swirling (can denature the peptide). Using non-bacteriostatic water for multi-use vials. Storing reconstituted peptide at room temperature. Reusing needles between vials (sterility failure).
Frequently Asked Questions
What water should I use to reconstitute peptides?+
Bacteriostatic water for multi-use research vials. We stock BAC water as an ancillary alongside the peptide catalogue.
How long does reconstituted peptide last?+
It depends on the compound class. As a general rule, store at 2–8 °C and use within the stability window listed on the product page. Refer to the CoA for batch-specific guidance.
Can I freeze reconstituted peptide?+
Repeated freeze-thaw cycles can degrade peptides. Reconstitute only what you need and keep the rest lyophilised at −20 °C until required.